微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pUT11 URA3 --> TRP1 converter |
|---|---|
| 商品货号： | TS161179 |
| Designations： | pUT11 URA3 --> TRP1 converter |
| Depositors： | FR Cross |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.6999998092651370 DESCRIPTION OF VECTOR: Intact vector size: 6.700 Type of vector: plasmid Cloning sites: Polylinker sites: Construction: pUC19 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): replicon: pMB1 marker(s): ampR, restriction site: EcoRI, SmaI gene disruption cassette: ura3::TRP1/kanR restriction site: SmaI Vector: pUT11 (plasmid) Construction: pUC19 Marker(s):TRP1,ampR,kanR Construct size (kb): 6.699999809265137 Features: gene disruption cassette: ura3::TRP1/kanR marker(s): ampR replicon: pMB1 restriction site: EcoRI, SmaI restriction site: SmaI |
| Applications： | YI-type (integrating) shuttle vector host modification marker swap vector URA3 --> TRP1 |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.6, 2.0; SmaI--3.8, 2.7. To convert the host phenotype from URA3 to TRP1, transform with the SmaI digested vector and select for Trp+ transformants. Some combinations of marker swap plasmids and target locus may result in relatively high reversion rates. In most but not all cases the frequencies of successful convertants are greater than 30%. A marker swap vector designed to change the S. cerevisiae host phenotype by one-step gene disruption of the URA3 gene with the TRP1 and kanR markers. When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance. Vector was constructed by replacing an internal StuI fragment of URA3 with a SmaI fragment containing the TRP1 and kanR coding sequences. TRP1 and URA3 are in the same orientation. |
| Media： | ATCC® Medium 1948: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 20 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cross FR. Marker swap plasmids: convenient tools for budding yeast molecular genetics. Yeast 13: 647-653, 1997. PubMed: 9200814 |
| Related Products： | component of:ATCC 87561 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |