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| 产品名称： | pJ3omega-MDR3 3.27 |
|---|---|
| 商品货号： | TS161216 |
| Designations： | pJ3omega-MDR3 3.27 |
| GenBank Number： | M23234 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein P glycoprotein 3/multiple drug resistance 3 |
| Vector： | Construct size (kb): 7.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.0 Tissue: liver Gene product: P glycoprotein 3/multiple drug resistance 3 MDR3 Target Gene: P glycoprotein 3/multiple drug resistance 3 |
| Insert Size (kb)： | 4.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.0, 3.5; BamHI--4.5, 3.0; AvaI--4.6, 2.0, 0.5, 0.4; EcoRI--3.6, 2.8, 1.2; PstI--2.9, 2.3, 0.9, 0.6, 0.5, 0.4. The insert contains the following restriction sites (nt positions from the 5 end): BamHI--987; XhoI--1246; EcoRI--2816. A full-length cDNA clone extending from nt -33 to +4002 relative to the translation initiation codon. The coding sequence is 3839 nt. When transfected into BRO melanoma cells, the sequence expresses a protein recognized by monoclonal antibodies but does not confer multiple drug resistance. Originally cloned using EcoRI linkers into the polylinker of lambdaZAP. Excised as a HindIII/XbaI fragment (sites from the polylinker) for insertion into pJ3omega. |
| References： | van der Bliek AM, et al. Sequence of mdr3 cDNA encoding a human P-glycoprotein. Gene 71: 401-411, 1988. PubMed: 2906314 Schinkel AH, et al. Characterization of the human MDR3 P-glycoprotein and its recognition by P-glycoprotein-specific monoclonal antibodies. Cancer Res. 51: 2628-2635, 1991. PubMed: 1673638 Piet Borst, personal communication |