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| 产品名称： | pEBA-4A8 |
|---|---|
| 商品货号： | TS161242 |
| Designations： | pEBA-4A8 |
| Depositors： | EW Miles |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.6400003433227500 Vector: pEBA-4A8 (plasmid) Promoters: Promoter Ptrc Construction: pEBA-10 Marker(s):TRP1,ampR Construct size (kb): 11.64000034332275 Features: primer site: 5-TTGACAATTAATCATCCGGC-3 promoter: Ptrc terminator: rrnBT1 terminator: rrnBT2 |
| Applications： | contains sequence |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--11.6; EcoRI--5.5, 1.1 (5x); PstI--11.6; EcoRI/PstI--4.4; 1.1 (6x). Overproduces alpha2 subunit of tryptophan synthase from Salmonella typhimurium. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Yang LH, et al. PCR mutagenesis and overexpression of tryptophan synthase from Salmonella typhimurium: on the roles of beta2 subunit Lys-382. Protein Expr. Purif. 8: 126-136, 1996. PubMed: 8812843 Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046 |
| Related Products： | component of:ATCC 87809 |
| Shipped： | freeze-dried |