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微信小章| 产品名称： | M27beta M27B, M27Beta |
|---|---|
| 商品货号： | TS161258 |
| Designations： | M27beta M27B, M27Beta |
| GenBank Number： | J03073 |
| Species： | Homo sapiens, human |
| Applications： | A stringency of 0.5 X SSC, 64 C was used routinely. |
| Vector： | Construct size (kb): 7.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 4.400000095367432 Tissue: DNA from mouse/human MOG-T hybrid line (X only) Gene product: DNA Segment, single copy DXS255 |
| Insert Size (kb)： | 4.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains 2 copies of a 2.2 kb insert containing the following restriction sites, separated by approximate kb: EcoRI- 0.4- HindIII- 0.1- BglII- 0.6- PvuII- 0.2- BglII- 0.9- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.4, 2.4; HindIII--4.55, 2.4, 0.55; PvuII--2.4, 2.4, 1.4, 1.3; BamHI--7.5; HindIII--7.5. The EcoRI RFLP shows significant linkage to Wiskott-Aldrich Syndrome (theta = 0.00, Z = 10.19). The 0.9 BglII/EcoRI fragment contains a 334 bp HaeIII/Sau3A fragment within which a deletion of about 3 kb occurred during cloning. The 334 bp HaeIII/Sau3A fragment contains 3 complete copies and a partial copy of a 26 bp repeat which includes a 10 bp perfect inverted repeat separated by 3 nt. EcoRI gives the best separation of alleles on 0.8% agarose gels. A stringency of 0.5 X SSC, 64 C was used routinely. |
| References： | Fraser NJ, et al. Multi-allelic RFLP for M27beta, an anonymous single copy genomic clone at Xp11.2-Xcen HGM9 provisional no. DXS255. Nucleic Acids Res. 15: 9616, 1987. PubMed: 2891116 Fraser NJ, et al. Isolation and characterization of a human variable copy number tandem repeat at Xcen-p11.2. Genomics 5: 144-148, 1989. PubMed: 2570025 Greer WL, et al. Linkage relationships of the Wiskott-Aldrich Syndrome to 10 loci in the pericentromeric region of the human X chromosome. Genomics 6: 568-571, 1990. PubMed: 2328995 Alitalo T, et al. Localization of the Aland Island Eye Disease locus to the pericentromeric region of the X chromosome by linkage analysis. Am. J. Hum. Genet. 48: 31-38, 1991. PubMed: 1985461 |