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| 产品名称： | pRBBP1-I cDNA |
|---|---|
| 商品货号： | TS161286 |
| Designations： | pRBBP1-I cDNA |
| GenBank Number： | S57153 |
| Species： | Homo sapiens, human |
| Depositors： | GA Otterson |
| Applications： | contains sequence retinoblastoma-binding protein 1, isoform I |
| Vector： | Construct size (kb): 7.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-4 Intact vector size: 2.870 Type of vector: plasmid Vector end: BamHI Vector end: HindIII Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): MCS: EcoRI...HindIII, -> promoter for in vitro transcription: T7, replicon: pMB1 marker(s): ampR, promoter for in vitro transcription: SP6, -> Cross references: DNA Seq. Acc.: X65303 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: RBBP1 Gene name: retinoblastoma-binding protein 1, isoform I Contains complete coding sequence?: Y Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 4.0 Cross references: DNA Seq. Acc.: S57153 Insert lengths(kb): 4.0 Gene product: retinoblastoma-binding protein 1, isoform I RBBP1 Target Gene: retinoblastoma-binding protein 1, isoform I |
| Insert Size (kb)： | 4.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.2; BamHI/HindIII--4.1, 3.1; EcoRI--3.0, 2.4, 1.2, 0.48; HindIII--7.2; SacI--3.2, 3.0, 0.8. |
| References： | Otterson GA, et al. Alternative splicing of the RBP1 gene clusters in an internal exon that encodes protential phosphorylation sites. Oncogene 8: 949-957, 1993. PubMed: 8455946 Fattaey AR, et al. Characterization of the retinoblastoma binding proteins RBP1 and RBP2. Oncogene 8: 3149-3156, 1993. PubMed: 8414517 |