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| 产品名称： | GnRHR |
|---|---|
| 商品货号： | TS161302 |
| Designations： | GnRHR |
| GenBank Number： | L03380 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein gonadotropin-releasing hormone receptor |
| Vector： | Construct size (kb): 5.559999942779541 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcDNA I Intact vector size: 4.000 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: HindIII BamHI BstXI EcoRI PstI EcoRV NotI XhoI SphI NsiI XbaI Polylinker sites: HindIII BamHI BstXI EcoRI PstI EcoRV BstXI NotI XhoI SphI NsiI XbaI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): enhancer: CMV promoter: CMV, -> promoter: T7, -> MCS: HindIII...XbaI, -> promoter: SP6, terminator: SV40 polyadenylation, -> replicon: polyoma ori replicon: SV40 replicon: pMB1 replicon: M13 ori, marker(s): supF, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GRHR Genomic copy number: unique Gene name: gonadotropin-releasing hormone receptor Contains complete coding sequence?: Y Tissue: pituitary Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 1.56 Cross references: DNA Seq. Acc.: L03380 Insert lengths(kb): 1.559999942779541 Tissue: pituitary Gene product: gonadotropin-releasing hormone receptor GRHR Target Gene: gonadotropin-releasing hormone receptor |
| Insert Size (kb)： | 1.560 |
| Media： | ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digest of the clone give the following sizes (kb): EcoRI--4.0, 1.55; BamHI--4.95, 0.6; HindIII--4.3, 0.8, 0.4; PstI--4.65, 0.9; PvuII--2.2, 1.8, 1.55. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.55; HindIII--0.42, 1.26; NdeI--1.06; NcoI--0.53; PstI--0.65; PvuII--0.70; SphI--1.42. Contains the complete coding sequence (nt 56-1042). The following primers can be used to amplify an 800 bp DNA fragment contained in the gene: upstream 5-GCTTGAAGCTCTGTCCTGGGA-3; downstream 5-CCTAGGACATAGTAGGG-3. When transfected into COS-7 cells, expresses a high-affinity receptor. Detects a 5.0 kb RNA transcript in human pituitary. Transcription from the T7 promoter gives the sense strand. |
| References： | Kakar SS, et al. Cloning, sequencing, and expression of human gonadotropin releasing hormone (GnRH) receptor. Biochem. Biophys. Res. Commun. 189: 289-295, 1992. PubMed: 1333190 |