微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pTOPO-ACS |
|---|---|
| 商品货号： | TS161331 |
| Designations： | pTOPO-ACS |
| GenBank Number： | AF263614 |
| Species： | Homo sapiens, human |
| Depositors： | JL Goldstein |
| Applications： | contains sequence acetyl-CoA synthetase |
| Vector： | Construct size (kb): 6.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCR2.1-TOPO Intact vector size: 3.908 Type of vector: plasmid Cloning sites: HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV BstXI NotI XhoI NsiI XbaI ApaI Polylinker sites: HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV BstXI NotI XhoI NsiI XbaI ApaI Features (with orientation and position when available): insert detection: lacZalpha, ->, 1-571 primer site: M13 reverse priming site, ->, 205-221 MCS: bases, ->, 234-357 promoter: T7, ->, 364-383 primer site: M13 forward (-20) priming site, ->, 391-406 primer site: M13 forward (-40) priming site, ->, 411-426 replicon: f1 origin, ->, 548-962 marker(s): kanR, ->, 1296-2090 marker(s): ampR, ->, 2108-2968 replicon: ColE1 origin, ->, 3113-3786 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene name: acetyl-CoA synthetase Contains complete coding sequence?: Y Strain: human HeLa Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 2.1 Cross references: Insert lengths(kb): 2.099999904632568 Gene product: acetyl-CoA synthetase Target Gene: acetyl-CoA synthetase |
| Insert Size (kb)： | 2.100 |
| Media： | 1065 plus ampicillin plus kanamycin
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BamHI/NotI--3.9, 2.1; EcoRI--3.8, 2.0.0.2; HindIII--4.1, 1.5,0.3. The pTOPO-ACS contains a 2.1 kb insert that encodes the full length cDNA for human acetyl CoA synthetase (ACS) (amino acids 1-701). THe plasmid was constructed in the following manner. Polymerase chain reaction was used to amplify the full length human ACS with twelve 5 prime untranslated region (UTR) nucleotides and 29 nucleotides of the 3 prime UTR. The PCR product was ligated directly into pCR 2.1-TOPO (Invitrogen). |
| References： | Luong A, et al. Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins. J. Biol. Chem. 275: 26458-26466, 2000. PubMed: 10843999 |