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| 产品名称： | pG32/T4 C41/U1/T4, aC41/U1-T4 |
|---|---|
| 商品货号： | TS161353 |
| Designations： | pG32/T4 C41/U1/T4, aC41/U1-T4 |
| GenBank Number： | J03493 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | A Tzagoloff |
| Vector： | Construct size (kb): 18.89999961853027 |
| Insert： | DNA: genomic Insert lengths(kb): 11.0 Gene product: 5-aminolevulinate dehydratase( aminolevulinate dehydratase, aminolevulinate,delta-,dehydratase, delta-aminolevulinate dehydratase, porphobilinogen synthase, 5-aminolevulinate dehydratase, delta aminolevulinate dehydratase) HEM2 |
| Insert Size (kb)： | 11.0 |
| Genotype： | MATa ura3-1 hem2-15 |
| Mating Type： | a |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (separated by approximate kb): HindIII- 1.0- BamHI- 0.3- HindIII- 3.2- HindIII- 1.9- EcoRI- 0.25- BamHI- 1.1- EcoRI. Restriction digests of the clone give the following sizes (kb): SmaI/SalI--8.6, 7.5, 2.7; SalI--16.0, 2.9; EcoRV--9.0, 6.2, 2.4, 1.0; XbaI--10.5, 4.1 (doublet). A 1.3 kb HindIII fragment from this insert is sufficient to complement either a hem2-15 (C41/U1) or a hem2-16 (N28/U2) mutation. The probe, pG32/T4, has been deposited in Escherichia coli RR1 as TS161353 and in Saccharomyces cerevisiae C41/U1 as ATCC 66314. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Myers AM, et al. Characterization of the yeast HEM2 gene and transcriptional regulation of COX5 and COR1 by heme. J. Biol. Chem. 262: 16822-16829, 1987. PubMed: 2445751 |