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| 产品名称： | RTG-P1 |
|---|---|
| 商品货号： | TS161368 |
| Organism： | Oncorhynchus mykiss, trout, rainbow |
| Tissue： | mixed; testis; ovary |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain SV-40 and CMV viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | male and female mixed |
| Applications： | The vector, pGL3-prMx1-Basic-Neo, expresses the firefly luciferase gene under the control of the promoter for the IFN-induced gene Mx1. The vector contains SV40 viral DNA sequences and the neomycin resistance gene. This cell line can be used to study the activation of the IFN pathway. The RTG-2 cell line (ATCC CCL-55) was transfected with a trout Mx1 promoter-luciferase construct and selected to produce a stable cell line, RTG-P1 PubMed: 15043947. The RTG-P1 reporter system constitutes an interesting tool to study the induction and regulation of IFN signaling in teleost fish. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | male and female mixed |
| Comments： | The RTG-2 cell line (ATCC CCL-55) was transfected with a trout Mx1 promoter-luciferase construct and selected to produce a stable cell line, RTG-P1 PubMed: 15043947. The vector, pGL3-prMx1-Basic-Neo, expresses the firefly luciferase gene under the control of the promoter for the IFN-induced gene Mx1. The vector contains SV40 viral DNA sequences and the neomycin resistance gene. Luciferase expression is stable and highly induced by polyinosinic:polycytidylic acid (poly I:C) in RTG-P1 cells PubMed: 15043947. This cell line can be used to study the activation of the IFN pathway. The RTG-P1 reporter system constitutes an interesting tool to study the induction and regulation of IFN signaling in teleost fish. |
| Complete Growth Medium： | Leibovitzs L-15 medium with 2 mM L-glutamine supplemented with 0.2 mg/ml G418 and 10% fetal bovine serum. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Protocol:
Interval: Maintain cultures at a cell concentration between 1 X 10(4) and 1 X 10(5) cells/sq. cm Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100% Temperature: 22.0°C |
| Population Doubling Time： | approximately 67 hours |
| Name of Depositor： | B Collet |
| References： | Collet B, et al. An Mx1 promoter-reporter system to study interferon pathways in rainbow trout. Dev. Comp. Immunol. 28: 793-801, 2004. PubMed: 15043947 |