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| 产品名称： | c1-37 |
|---|---|
| 商品货号： | TS161406 |
| Designations： | c1-37 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Vector： | Construct size (kb): 44.59999847412109 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy D20S8 Alleles: A1, A2, B1, B3, B2 |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--uncut; HindIII--22, 11, 5.9, 4.5, 1.5, 1.0; EcoRI--20, 9.8, 7.1, 3.5, 2.5, 1.1, 0.9, 0.7; PstI--7.1, 4.2, 3.3, 2.7, 2.2, 1.9, 1.7, 1.3, 1.1, 1.0, 0.9 + smaller; BamHI--9.8, 8.7, 7.9, 7.1, 3.1, 2.5, 2.5, 1.1. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Constant fragment sizes are 3.4, 2.3, 2.1, 1.44, 1.29, 1.16, 1.03, 0.94, and 0.84 kb. The same locus is revealed with a EcoRI/HindIII digest. This locus is in equilibrium with that detected by p37-1-34; the compound locus has a PIC of 0.54. A 20 kb EcoRI fragment probe can be obtained by digesting cosmid DNA with EcoRI and cutting the largest (20 kb) fragment out of the agarose gel. This is single-copy and gives good results with standard hybridization and washing conditions. |
| References： | Litt M, et al. A polymorphic locus on the long arm of chromosome 20 defined by two probes from a single cosmid. Hum. Genet. 73: 340-345, 1986. PubMed: 3017843 Michael Litt, personal communication |