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微信小章| 产品名称： | pSGT |
|---|---|
| 商品货号： | TS161437 |
| Designations： | pSGT |
| Species： | Homo sapiens, human |
| Depositors： | B Thorens |
| Vector： | Construct size (kb): 5.449999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.47000002861023 Tissue: hepatocellular carcinoma Hep G2 cell line Gene product: solute carrier family 2 (facilitated glucose transporter), member 1(glucose transporter 1) GLUT |
| Insert Size (kb)： | 2.470 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.3, 0.38; BamHI--3.0, 2.45; HindIII--4.65, 0.58; PstI--3.0, 1.65, 0.54, 0.21; ClaI--uncut. The cDNA insert extends from a synthetic BamHI site introduced at the HaeII site at position -4 to the BamHI site located at position 2469. |
| References： | Mueckler M, et al. Sequence and structure of a human glucose transporter. Science 229: 941-945, 1985. PubMed: 3839598 Mueckler M, Lodish HF. The human glucose transporter can insert posttranslationally into microsomes. Cell 44: 629-637, 1986. PubMed: 3004742 Sarkar HK, et al. Expression of the human erythrocyte glucose transporter in Escherichia coli. Proc. Natl. Acad. Sci. USA 85: 5463-5467, 1988. PubMed: 2840662 Bernard Thorens, personal communication |