微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pDG1 |
|---|---|
| 商品货号： | TS161438 |
| Designations： | pDG1 |
| Depositors： | Allelix Biopharmaceuticals, Inc., G Strachan, Allelix Biopharmaceuticals, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 |
| Vector Information： | Size (kb): 12.1999998092651400 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Vector end: BamHI Vector end: EcoRI Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873 Vector: pDG1 (cosmid) Construction: pHC79, pBB116 Marker(s):ampR,argB Construct size (kb): 12.19999980926514 Features: marker(s): ampR, argB replicon: pMB1 |
| Applications： | contains sequence ornithine carbamoyltransferase integrating vector shuttle vector vector for constructing genomic libraries vector permitting positive selection for integration |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--9.4, 1.5, 1.3; EcoRI/BamHI--6.3, 5.9; PstI--10.2, 2.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. This is an integrative, positive selection vector conferring arginine prototrophy on an auxotrophic host such as Aspergillus niger 350(-)52 (ATCC 20739) (argB-). The argB BamHI/EcoRI fragment contains the following sites (separated by approximate kb): BamHI- 0.8- SalI- 0.4- HindIII- 1.4- SalI- 0.1- HindIII- 0.7- SalI- 0.1- HindIII- 0.7- SalI- 0.7- SalI- 0.5- EcoRI. This was constructed by inserting the 5.0 kb EcoRI/HindIII fragment from pBB116 containing argB into pHC79 cut with EcoRI + HindIII. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Buxton FP, et al. Aspergillus niger transformation system. US Patent 4,885,249 dated Dec 5 1989 Berse B, et al. Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulans. Gene 25: 109-117, 1983. PubMed: 6363209 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |