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| 产品名称： | Hulambda4x-8 |
|---|---|
| 商品货号： | TS161441 |
| Designations： | Hulambda4x-8 |
| Species： | Homo sapiens, human |
| Depositors： | C Caskey |
| Vector： | Construct size (kb): 62.0 |
| Insert： | DNA: genomic Insert lengths(kb): 16.79999923706055 Tissue: lymphoblast GM1202 (49,XXXXY) cell line Gene product: hypoxanthine phosphoribosyltransferase HPRT Alleles: A1, A2, A3 |
| Insert Size (kb)： | 16.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--19, 13, 3.8. Sequences at the 3 end of the insert apparently rearranged during cloning and include an internal artificial EcoRI site. The region between bases -170 and -235 of the published sequence is highly GC rich (91%). The 5 end of the insert is 430 bp upstream of ATG of exon 1. The insert contains exon 1, but not exon 2. The insert contains no canonical promoters or enhancers, but the region between -234 and -49 is necessary for transcription. |
| References： | Patel PI, et al. Fine structure of the human hypoxanthine phosphoribosyltransferase gene. Mol. Cell. Biol. 6: 393-403, 1986. PubMed: 3023844 |