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| 产品名称： | pKLBglIM250-10 |
|---|---|
| 商品货号： | TS161478 |
| Designations： | pKLBglIM250-10 |
| Species： | Bacillus globigii Migula |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | produces protein modification methylase BglI produces protein restriction endonuclease BglI |
| Vector： | Construct size (kb): 3.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 1.235999941825867 Gene product: modification methylase BglI bglR Target Gene: modification methylase BglI, restriction endonuclease BglI |
| Insert Size (kb)： | 1.236 |
| Media： | ATCC Medium 1065 (see below) plus ampicillin (100 ug/ml) plus kanamycin (50 ug/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of pKL143R100-1 give the following sizes (kb): BamH/EcoRI--4.0; EcoRI--4.0; EcoRI/HindIII--2.7, 1.3. Restriction digests of pKLBglIM250-10 give the following sizes (kb): BamHI/EcoRI--7.2, 1.5; EcoRI--8.6; EcoRI+BamHI--4.3 (doublet). The intensity of these bands is less than those derived from pKL143R100-1. |
| References： | Lunnen KD, Wilson GG. Method for producing the BglI restriction endonuclease and methylase. US Patent 5,366,882 dated Nov 22 1994 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |