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| 产品名称： | pEX11 |
|---|---|
| 商品货号： | TS161523 |
| Designations： | pEX11 |
| Depositors： | BA Van Der Zeijst |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.8000001907348630 Vector: pEX11 (plasmid) Promoters: Promoter lambda PR Construction: pEX1, oligonucleotide Marker(s):ampR Construct size (kb): 5.800000190734863 Features: marker(s): ampR promoter: lambda PR replicon: pMB1 terminator: phage fd enhancer: none |
| Applications： | expression vector vector for constructing cDNA libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.8; BamHI--5.8; PstI--5.8; EcoRI/EcoRV--4.3, 1.9; SalI--5.8. This strain is resistant to 100 ug/ml ampicillin. The recombinant fusion protein generated using this vector is not solubilized in Triton X-100 lysis procedures and is thus easily purified, resistant to proteolysis, and easily detected by direct immunoscreening. This is one of three bacterial expression vectors (pEX11, TS161523; pEX12, ATCC 37709; and pEX13, ATCC 37710) which differ by translational reading frames and which express cro-lacZ fusion proteins regulated by the PR promoter of bacteriophage lambda. This was constructed from pEX1 by the ligating a synthetic oligonucleotide into pEX1 cut with XmaI and BamHI. The oligonucleotide added several unique restriction sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Kusters JG, et al. Improvement of the cloning linker of the bacterial expression vector pEX. Nucleic Acids Res. 17: 8007, 1989. PubMed: 2678010 Stanley KK, Luzio JP. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO J. 3: 1429-1434, 1984. PubMed: 6086324 Bernard A Van Der Zeijst, personal communication |
| Shipped： | frozen |