微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pLGdelta178 |
|---|---|
| 商品货号： | TS161524 |
| Designations： | pLGdelta178 |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.0600004196167000 Vector: pLGdelta178 (plasmid) Promoters: Promoter truncated CYC1 promoter (no UAS element) Construction: pBR322 Marker(s):URA3,ampR Construct size (kb): 10.0600004196167 Features: marker(s): URA3 marker(s): ampR promoter: truncated CYC1 promoter (no UAS element) replicon: 2 micron ori replicon: pMB1 coding sequence: lacZ |
| Applications： | YE-type (episomal) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--11.0; HindIII--8.7, 2.2; EcoRI--4.75, 3.45, 2.4. This is one of three lacZ reporter plasmids that lack UAS promoter elements (TS161524, 87510 & 87513). They differ by polylinker sites. DNA fragments can be inserted into these plasmids to assay UAS (upstream activation site) activity. The vector was constructed by Lenny Guarente. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Guarente L, et al. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae. Cell 36: 503-511, 1984. PubMed: 6319028 |
| Related Products： | component of:ATCC 87539 |
| Shipped： | freeze-dried |