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| 产品名称： | pQL1 |
|---|---|
| 商品货号： | TS161541 |
| Designations： | pQL1 |
| Depositors： | GJ Heidecker |
| Biosafety Level： | 2 |
| Vector Information： | Size (kb): 8.0000000000000000 Vector: pQL1 (plasmid) Construction: pACYC184, pKK232-8, kanR Marker(s):ampR,cmlR,kanR,rbR Construct size (kb): 8.0 Features: marker(s): ampR marker(s): cmlR marker(s): kanR (neoR, rbR) other: Haemophilus-specific uptake sequence replicon: p15A replicon: pMB1 MCS: EcoRI...HindIII stop codons: 3 reading frames transcription terminator: T1 transcription terminator: T1 T2 |
| Applications： | contains sequence |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.7, 0.3; BamHI--8.0; HindIII--8.0; PstI--3.5, 3.4, 0.9; SalI--4.6, 3.4. Do not incubate with carbon dioxide when using ribostamycin. The TSTE element is a 2.2 kb BamHI fragment that includes neoR from Tn5 flanked by Haemophilus-specific uptake sequences. Ribostamycin resistance is useful to select for the presence of neoR in both E. coli (40 ug/ml) and H. influenzae (15 ug/ml). Copy number is estimated at 6-13 per cell in both H. influenzae and E. coli. J.M. Musser |
| Media： | ATCC® Medium 814: GC Agar/Broth Medium |
| Growth Conditions： | Temperature: 37.0°C Protocol: 5% CO2 |
| References： | Heidecker GJ, et al. Construction of an ori cassette for adapting shuttle vectors for use in Haemophilus influenzae. Gene 150: 141-144, 1994. PubMed: 7959040 Terrence L Stull, personal communication |
| Shipped： | freeze-dried |