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购买须知
| 产品名称： | Mus musculus |
|---|---|
| 商品货号： | TS161545 |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| Agent： | Mus musculus |
| Strain： | monoclonal antibody against FOS peptide (359-378) LA 054 |
| Common Name： | mouse |
| Applications： | Source and Sex of Animals: Male 129GIX+ mice were used for immunization. Cross-reactivity: In immunoblot screening a protein of approximately 55 kd is detected in a fibroblast cell line transformed by fes, sis, and scr. Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Source and Sex of Animals: Male 129GIX+ mice were used for immunization. |
| Product Format： | freeze-dried Titer: Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay. |
| Storage Conditions： | Vials may be stored at 2-8°C. |
| Comments： | Immunogen: Synthetic peptide RKGSSSNEPSSDSLSSPTLL(C) (residues 359-378 of the FOS sequence) coupled to keyhole limpet hemocyanin Source and Sex of Animals: Male 129GIX+ mice were used for immunization. Fusion Partners: 1.3 x 10(8) nucleated spleen cells with 2 x 10(7) SP2/0 non-secreting myeloma cells Cross-reactivity: In immunoblot screening a protein of approximately 55 kd is detected in a fibroblast cell line transformed by fes, sis, and scr. Immunization Schedule: Approximately 70 mg of coupled peptide in 300 ml of a 1:1 mixture of phosphate buffered saline pH 7.4 and complete Freunds adjuvant administered intraperitoneally on day 0; followed by an equal amount of conjugate in 300 ml of a 1:1 mixture of phosphate buffered saline pH 7.4 and 10 mg/ml alum administered intraperitoneally on day 14; followed by 70 mg of coupled peptide in 200 ml of phosphate buffered saline pH 7.4 administered intravenously on day 51. The spleen was removed on day 56. Preparation: Monoclonal antibody is in ascites fluid collected from 2-06-87 to 7-06-87. The ascites fluid was ammonium sulfate precipitated, reconstituted, filter sterilized, aliquoted 1 ml per vial, and lyophilized. The sterility is not guaranteed. Resuspend in 1 ml distilled water. Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay Vials may be stored at 2-8°C. IgG1 |
| Effect on Host： | Approximately 1:100,000 against the unconjugated synthetic peptide in an indirect enzyme-linked immunosorbent assay |
| Recommended Host： | Source and Sex of Animals: Male 129GIX+ mice were used for immunization. |
| Cryopreservation： | Storage temperature: Vials may be stored at 2-8°C. |