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| 产品名称： | Canid herpesvirus 1 |
|---|---|
| 商品货号： | TS161566 |
| Classification： | Herpesviridae, Varicellovirus |
| Agent： | Canid herpesvirus 1 |
| Strain： | F 205 (Carmichael) |
| Common Name： | Canine herpesvirus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C |
| Comments： | Derived by adaptation of primary cell culture product ATCC VR-647 to MDCK cells (ATCC CCL-34). ATCC VR-647 and TS161566 have not been compared for sequence or infectivity in primary cell culture and tissue culture. Chloroform/ether sensitive, not known to hemagglutinate, sensitive to pH below 6.5 and heat labile. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | MDCK (ATCC CCL-34) |
| Growth Conditions： | Temperature: 37° Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-4 days |
| Name of Depositor： | JH Gillespie, New York State Veterinary College, Cornell University |
| Source： | Kidney from 2-3 weeks old puppy |
| References： | Carmichael LE, et al. Clinical and pathologic features of a fatal viral disease of newborn pups. Am. J. Vet. Res. 26: 803-814, 1965. PubMed: 5892835 Carmichael LE, et al. Identification of a cytopathogenic agent infectious for puppies as a Canine herpesvirus. Proc. Soc. Exp. Biol. Med. 120: 644-650, 1965. PubMed: 4221827 |