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| 产品名称： | p36/6 |
|---|---|
| 商品货号： | TS161661 |
| Designations： | p36/6 |
| GenBank Number： | M14056 |
| Species： | Bos taurus, cow |
| Depositors： | BF Tack |
| Vector： | Construct size (kb): 4.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.600000023841858 Tissue: MDBK kidney cell line Gene product: annexin II( annexin II, calpactin I heavy chain, lipocortin II, calpactin I, heavy polypeptide (p36)) Cal1h |
| Insert Size (kb)： | 1.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains the following restriction sites (in bp from the 5 end): BclI--99; BglII--964; HincII--223; PstI--472; ScaI--1336; SmaI--1172; XhoI--1332. Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.6; PstI--2.75, 1.25, 0.55; SmaI--4.6. Contains a complete coding sequence, with 5 and 3 untranslated regions. The open reading frame is 1014 bp. At the amino acid level, there are 6 amino acid changes between the bovine and human sequences. |
| References： | Kristensen T, et al. Primary structure of bovine calpactin I heavy chain (p36), a major cellular substrate for retroviral protein-tyrosine kinases: homology with the human phospholipase A2 inhibitor lipocortin. Biochemistry 25: 4497-4503, 1986. PubMed: 2945590 Brian F Tack, personal communication |