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| 产品名称： | pHF12-62 12-62, DOSLC6, p12-62 |
|---|---|
| 商品货号： | TS161732 |
| Designations： | pHF12-62 12-62, DOSLC6, p12-62 |
| Species： | Homo sapiens, human |
| Depositors： | RL White |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 0.949999988079071 Gene product: DNA Segment, single copy D18S1 Alleles: A1, A2 |
| Insert Size (kb)： | 0.950 |
| Media： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.2; HindIII--5.2; SalI--5.2; HindIII/XhoI--5.2; XhoI--uncut; HindIII/SalI--3.6, 1.7; HindIII/EcoRI--3.6, 1.5. The BamHI insert site was lost during construction. The HindIII/XhoI double digest releases the insert plus about 0.5 kb of the vector. Enzyme(s) not detecting polymorphism: MboI, MspI, EcoRI, HindIII, PvuII, BglII, BclI, HincI, PstI, SstI, XmaI, AccI, StuI, RsaI, EcoRV, ApaI, NcoI, TthI, XmnI, BstXI. |
| References： | Barker D, et al. Restriction sites containing CpG show a higher frequency of polymorphism in human DNA. Cell 36: 131-138, 1984. PubMed: 6198090 Naylor SL, et al. DNA polymorphic loci mapped to human chromosome 3, 5, 9, 11, 17, 18, and 22. Proc. Natl. Acad. Sci. USA 81: 2447-2451, 1984. PubMed: 6585809 |