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| 产品名称： | CL4-1 T-ALK |
|---|---|
| 商品货号： | TS161779 |
| Designations： | CL4-1 T-ALK |
| GenBank Number： | U20165 |
| Species： | Homo sapiens, human |
| Applications： | contains sequence activin A receptor type II-like kinase |
| Vector： | Construct size (kb): 6.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: XhoI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: ACVRLK* Gene name: activin A receptor type II-like kinase Contains complete coding sequence?: Y Tissue: placenta, male caucasian fetus Type of DNA: cDNA Insert 5 end: XhoI Insert 3 end: EcoRI Insert size (kb): 3.3 Cross references: DNA Seq. Acc.: D50516 Nucleotides 1-3167 of the insert correspond to nucleotides 373-3539 of D50516 with 100% identity. DNA Seq. Acc.: U20165 Nucleotides 1-3167 of the insert correspond to nucleotides 1-3167 of U20165. Insert lengths(kb): 3.299999952316284 Tissue: placenta, male caucasian fetus Gene product: activin A receptor type II-like kinase ACVRLK* Target Gene: activin A receptor type II-like kinase |
| Insert Size (kb)： | 3.300 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.3, 3.0; PstI--3.1, 2.4, 0.8; BgII--6.3; XbaI--6.3; PvuII--2.6, 2.2, 1.5. Insert contains the following restriction sites (approximate kb from the 5 end): BglII--2.26; HincII--2.48; NcoI--0.08, 3.12; PstI--0.05, 0.84; PvuII--2.00; StuI--2.84; XbaI--0.63. Insert contains a poly-T cloning artifact between the XhoI site and the 5 end of the gene. |
| References： | Kawabata M, et al. Cloning of a novel type II serine/threonine kinase receptor through interaction with the type I transforming growth factor-beta receptor. J. Biol. Chem. 270: 5625-5630, 1995. PubMed: 7890683 |