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| 产品名称： | pRP9 |
|---|---|
| 商品货号： | TS161783 |
| Designations： | pRP9 |
| Depositors： | E De Rossi, G Riccardi |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.9000000953674320 Vector: pRP9 (plasmid) Construction: pLM6, pUC19 Marker(s):cmlR Construct size (kb): 2.900000095367432 Features: marker(s): cmlR replicon: pBC1 MCS: EcoRI...HindIII |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9; EcoRI--2.9; HhaI--2.9. In B. stearothermophilus, pRP9 does not undergo rolling circle replication (RCR); in B. subtilis, RCR is detected. |
| Media： | ATCC® Medium 2224: LB + chloramphenicol (5 mcg/ml) |
| Growth Conditions： | Temperature: 37.0°C |
| References： | De Rossi E, et al. New shuttle vector for cloning in Bacillus stearothermophilus. Res. Microbiol. 145: 579-583, 1994. PubMed: 7871236 |
| Shipped： | freeze-dried |