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产品简介
购买须知
| 产品名称： | cYN5.48 |
|---|---|
| 商品货号： | TS161790 |
| Designations： | cYN5.48 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Vector： | Construct size (kb): 48.0 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy D5S81 Alleles: A1, A2 |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--23.0, 8.3, 7.0, 2.3; EcoRI--20.0, 8.5 (doublet), 2.5, 2.3, 1.4, 0.98; HindIII--7.0, 6.0, 5.4, 4.2, 3.9, 3.3, 3.2, 2.5, 2.0, 1.8, 1.7, 1.6, 1.0, 0.98; BglII--15.0, 7.2, 6.6, 6.0, 5.4, 3.2, 2.2, 1.6, 1.5, 1.4; SalI--26.0, 23.1, 2.7. Codominant segregation demonstrated in 60 families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: PstI, MspI. |
| References： | Raymond L White, personal communication |