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| 产品名称： | p426 MET25 |
|---|---|
| 商品货号： | TS161808 |
| Designations： | p426 MET25 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.3379998207092290 DESCRIPTION OF VECTOR: Intact vector size: 6.338 Type of vector: plasmid Cloning sites: SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS426 (ATCC 77107), MET25 promoter Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): URA3, ->, 200-1300 terminator: CYC1, ->, 2004-2264 MCS: XhoI...SpeI, ->, 2265-2322 promoter for expression: MET25, marker(s): ampR, replicon: 2 micron, ->, 4868-6208 Vector: p426 MET25 (plasmid) Promoters: Promoter for expression MET25 Construction: pRS426 (ATCC 77107), MET25 promoter Marker(s):URA3,ampR Construct size (kb): 6.337999820709229 Features: marker(s): URA3 marker(s): ampR promoter for expression: MET25 replicon: 2 micron MCS: XhoI...SpeI terminator: CYC1 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.4; EcoRV--4.7, 1.7; PstI--4.5, 1.9. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |