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| 产品名称： | Charon BS(+) |
|---|---|
| 商品货号： | TS161814 |
| Designations： | Charon BS(+) |
| Depositors： | A Swaroop |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 43.0999984741210900 Vector: Charon BS(+) (phage, lambda - replacement) Promoters: Promoter for in vitro transcription T3 Construction: Charon 15, pBluescript KS M13(+) Marker(s):ampR Construct size (kb): 43.09999847412109 Features: insert detection: lacZ marker(s): ampR other: left arm other: pBluescript KS + other: right arm promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: f1 replicon: pMB1 MCS: NotI...KpnI MCS: SacI, NotI |
| Applications： | expression vector lambda vector allowing conversion to plasmid vector containing primer sites useful for sequencing vector for constructing cDNA libraries vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--27.0, 21.0, 6.4; EcoRI--30.0, 20.0; SalI--32.0, 11.0, 9.2, 0.26; XhoI--31.0, 13.5, 7.2. Vector for high-efficiency directional cloning. Clones can be converted to plasmids by recircularization after a NotI digest. Charon BS(-) (ATCC 77446) and Charon BS(+) (TS161814) differ in the orientation of the M13 origin of replication. |
| References： | Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160 |
| Shipped： | freeze-dried |