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| 产品名称： | pMR101 |
|---|---|
| 商品货号： | TS161953 |
| Designations： | pMR101 |
| Depositors： | L Regan, M Munson |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JM101 |
| Vector Information： | Size (kb): 4.0999999046325680 DESCRIPTION OF VECTOR: Intact vector size: 4.100 Type of vector: phagemid Cloning sites: NcoI BamHI Polylinker sites: Other unique sites: EcoNI, SalI, BstEII, PstI, BglII, XbaI Construction: pTM201/NS3-3, pET8c, pACYC177, pET11d Host range: Escherichia coli Features (with orientation and position when available): replicon: M13, ->, 11-467 replicon: p15A, ->, 1585-1587 marker(s): kanR, ->, 2179-2994 operator: lac, promoter for expression: T7 (phi10), Vector: pMR101 (phagemid) Promoters: Promoter for expression T7 (phi10) Construction: pTM201/NS3-3, pET8c, pACYC177, pET11d Marker(s):kanR Construct size (kb): 4.099999904632568 Features: marker(s): kanR operator: lac promoter for expression: T7 (phi10) replicon: M13 replicon: p15A |
| Applications： | expression vector vector permitting production of single-stranded DNA |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.1; BstEII/PstI--3.0, 1.08; SalI--4.1. There is an error in the reference in figures 1 and 2. The restriction sites, XbaI and BglII, are drawn in reverse order on the maps for pMR101. Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell. If used in an Escherichia coli strain that expresses T7 polymerase under the control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result in high levels of recombinant protein production. Use of 5NcoI and 3BamHI cloning sites is similar to that of other expression systems, which facilitiates transfer of genes into these pMR vectors. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Munson M, et al. ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene 144: 59-62, 1994. PubMed: 8026759 Mary Munson, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |