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微信小章| 产品名称： | phigf1 |
|---|---|
| 商品货号： | TS161968 |
| Designations： | phigf1 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.659 Tissue: liver Gene product: insulin-like growth factor 1 IGF1 Alleles: A1, A2 |
| Insert Size (kb)： | 0.659 |
| Related Products： | 59944 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert contains the following restriction sites (nt from the 5 end): RsaI--323; BamHI--420. Restriction digests of the clone give the following sizes (kb): HindIII--4.6; EcoRI--4.6; AvaI--4.6; BamHI--3.5, 1.05; PstI--3.9, 0.68. The insert extends from the second nucleotide of the codon for amino acid -15 of the signal peptide to the poly(A) tract. Detects genomic EcoRI fragments of 8.0, 6.6, and 4.3 kb in human cell lines, and fragments of 6.7, 2.1, and 1.9 kb in mouse. This probe also reveals a HindIII polymorphism which appears to be linked to the PvuII polymorphism. The vector pKT218 is pBR322 with an approximate 600 bp deletion of the ampR gene. The deletion starts near the beginning of the amp gene but is distal to the amp promoter and initiation ATG site and ends at the PstI site. The sequence from the ATG start of the amp gene to the PstI site is :
ATG AGT ATT CAA GCT GCA G This construct comes as 200 ng of purified plasmid DNA. It is also available already in E. coli as ATCC# 59944. |
| References： | Bell GI, et al. Sequence of a cDNA clone encoding human preproinsulin-like growth factor II. Nature 310: 775-777, 1984. PubMed: 6382021 Tricoli JV, et al. Localization of insulin-like growth factor genes to human chromosomes 11 and 12. Nature 310: 784-786, 1984. PubMed: 6382024 Morton CC, et al. Human genes for insulin-like growth factors I and II and epidermal growth factor are located on 12q22----q24.1, 11p15, and 4q25----q27, respectively. Cytogenet. Cell Genet. 41: 245-249, 1986. PubMed: 3486749 Graeme I Bell, personal communication |