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微信小章| 产品名称： | CA-77 |
|---|---|
| 商品货号： | TS162036 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | thyroid C cells |
| Product Format： | frozen |
| Morphology： | neuronal-like |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | medullary thyroid carcinoma |
| Applications： | neuronal differentiation and peptide secretion studies |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | CA77 was subcloned from a serially passaged collagenase and trypsin digestion of a transplantable rat medullary thyroid carcinoma of the CA tumor series, serum free media was used to restrict fibroblast outgrowth, after 23 subcultures serum was added to media |
| Genes Expressed： | calcitonin gene-related protein (CGRP) |
| Comments： | CA77 cells have a neuronal-like phenotype, neurite extension is enhanced by growth on laminin substrate. Cells have a neural crest derived lineage, which makes them a useful model for differentiation studies; expresses calcitonin gene-related peptide (CGRP) and other neuropeptides, which along with the serotonergic neuronal-like phenotype, makes them a useful model for gene expression and other molecular studies. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium renewal: Every 2 to 3 days |
| Cryopreservation： | Complete growth medium, 95% supplemented with 5% DMSO |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | B Sanborn |
| Year of Origin： | 1980 |
| References： | Muszynski M, et al. Glucocorticoids stimulate the production of preprocalcitonin-derived secretory peptides by a rat medullary thyroid carcinoma cell line. J. Biol. Chem. 258(19): 11678-83, 1983. PubMed: 6311819 Birnbaum RS, et al. Identification of procalcitonin in a rat medullary thyroid carcinoma cell line. J. Biol. Chem. 259(5): 2870-4, 1984. PubMed: 6199352 Russo AF, et al. Neuronal properties of a thyroid C-cell line: partial repression by dexamethasone and retinoic acid. Mol. Endocrinol. 6(2): 207-18, 1992. PubMed: 1569964 Birnbaum RS, et al. Biosynthesis of calcitonin by a rat medullary thyroid carcinoma cell line. J. Biol. Chem. 261(2): 699-703, 1986. PubMed: 3941098 Durham PL, et al. Repression of the calcitonin gene-related peptide promoter by 5-HT1 receptor activation. J. Neurosci. 17(24): 9545-53, 1997. PubMed: 9391009 Wood JL, et al. Autoregulation of cell-specific MAP kinase control of the tryptophan hydroxylase promoter. J. Biol. Chem. 276(24): 21262-71, 2001. PubMed: 11283010 Viney TJ, et al. Regulation of the cell-specific calcitonin/calcitonin gene-related peptide enhancer by USF and the Foxa2 forkhead protein. J. Biol. Chem. 279(48): 49948-55, 2004. PubMed: 15385550 Park KY, et al. Control of the calcitonin gene-related peptide enhancer by upstream stimulatory factor in trigeminal ganglion neurons. J. Biol. Chem. 283(9):5 441-51, 2008. PubMed: 18167349 |