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| 产品名称： | pADbeta |
|---|---|
| 商品货号： | TS162057 |
| Designations： | pADbeta |
| Depositors： | GR Macgregor |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.1999998092651370 Vector: pADbeta (plasmid) Promoters: Promoter Ad2 major late Construction: pUC19, pC4AUG beta-gal, p91023(B) Marker(s):ampR Construct size (kb): 7.199999809265137 Features: insert detection: lacZ marker(s): ampR promoter: Ad2 major late replicon: pMB1 terminator: SV40 late |
| Applications： | contains easily purifiable cassette(s) for construction reporter construct vector for other uses vector permitting visual detection of activity in colonies |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--3.9, 3.3; EcoRI/HindIII--3.9, 2.7, 0.6; NotI--3.6 (doublet); BamHI--7.0, 0.2. Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. Designed primarily as an enhancer trap vector. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. The lacZ gene may be excised by NotI and replaced with alternative sequences. The order of the major features in this plasmid is: pUC19 - EcoRI - adenovirus 2 major late promoter/tripartite leader/splice signal - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. |
| Media： | ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | MacGregor GR, Caskey CT. Construction of plasmids that express E. coli beta-galactosidase in mammalian cells. Nucleic Acids Res. 17: 2365, 1989. PubMed: 2495524 |
| Shipped： | freeze-dried |