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| 产品名称： | pK18mobsacB |
|---|---|
| 商品货号： | TS162074 |
| Designations： | pK18mobsacB |
| Depositors： | A Schaefer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.7
Vector: pK18mobsacB (plasmid)
Construction: pK18, pSUP102 (RP4 mob), sacB
Marker(s):kanR,sacB
Construct size (kb): 5.7
Features: insert detection: lacZ marker(s): kanR marker(s): sacB (sucrose sensitivity) replicon: oriT replicon: oriV MCS: EcoRI...HindIII |
| Applications： | integrating vector mobilizable vector produces protein levansucrase vector containing primer sites useful for sequencing vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.7; HindIII--5.7; PstI--5.7. After mobilization, the plasmid can be maintained by integration into the host chromosome via homologous recombination. Excision of the intervening plasmid sequence by a double cross-over event can be faciliated by selection on medium containing 10 percent sucrose. The sacB gene has been modified to eliminate the HindIII and EcoRI sites in the coding region. Differs from pK19mobsacB (ATCC 87098) only in the orientation of the polylinker. Cloning vector allowing mobilization into a wide range of Gram- and Gram+ bacteria. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983. Schafer A, et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145: 69-73, 1994. PubMed: 8045426 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen E. coli containing the plasmid. |