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| 产品名称： | pKB750 |
|---|---|
| 商品货号： | TS162083 |
| Designations： | pKB750 |
| GenBank Number： | M10431 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | OmniGene, Inc., KC Backman, OmniGene, Inc. |
| Applications： | produces protein chorismate mutase-P-prephenate dehydrogenase |
| Vector： | Construct size (kb): 7.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic Insert lengths(kb): 1.379999995231628 Gene product: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase aroF Target Gene: chorismate mutase-P-prephenate dehydrogenase |
| Insert Size (kb)： | 1.380 |
| Media： | ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/KpnI--4.4, 1.4, 1.2; EcoRV--4.4, 2.6; EcoRI--4.4, 2.6. Expression from the aroF promoter results in an aroF-pheA fusion protein. Insert contains aa 1-351 of the aroF coding sequence and the complete pheA coding sequence. The construct contains a weak attenuator sequence at the 5 end of the pheA coding sequence. Production of phenylalanine is enhanced when the pheA structural gene is introduced as part of a synthetic operon, rather than as a single deregulated structural gene. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Backman KC. Method of biosynthesis and cells therefor. US Patent 4,839,286 dated Jun 13 1989 Backman KC. Enzyme deregulation. US Patent 4,753,883 dated Jun 28 1988 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |