宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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XLIMPV1B

货号 TS162186
中文名称 null
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产品简介
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产品名称: XLIMPV1B
商品货号: TS162186
Designations: XLIMPV1B
GenBank Number:

L36339

Species: Xenopus laevis, frog, South African clawed
Depositors: E Hartmann
Vector:
Construct size (kb): 4.699999809265137
Insert:
DNA: cDNA
Insert lengths(kb): 1.672999978065491
Tissue: ovary
Gene product: importin 60-1 variant b IMPV1B*
Insert Size (kb): 1.673
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--3.0, 1.7; EcoRI--4.4, 0.3; PstI--3.3, 0.7, 0.5, 0.3; PvuII--2.5, 1.6, 0.65.
The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--0.28, 0.40; PstI--0.43, 1.10, 1.36; PvuII--1.26.
Excising the insert with BamHI/XhoI will add approximately 20 bp from the MCS of the vector sequence to the 5 end of the insert.
References:

Gorlich D, et al. Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79: 767-778, 1994. PubMed: 8001116