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微信小章| 产品名称： | Clostridioides difficile (Prevot) Lawson et al. |
|---|---|
| 商品货号： | TS162204 |
| Deposited As： | Clostridium difficile (Hall and OToole) Prevot |
| Strain Designations： | 14797-2 |
| Isolation： | stool of a 13-year-old female with fulminant hepatitis and severe encephalopathy, Michigan, 1977 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Shipping Information： | Frozen |
| Preceptrol®： | no |
| Genotype： | Toxinotype: 0 |
| Type Strain： | no |
| Comments： | Preparation of Clostridium difficile strain 14797-2 ATCC® 700792-FZx99, frozen for ease of use. Lot specific titers are available upon request. Toxinotype 0 Ribotype 005 Presence of tcdA and tcdB genes confirmed by PCR. Binary toxin gene cdtB not amplified by PCR This strain was isolated from what may possibly be the first reported case of Clostridium difficile-associated diarrhea (CDAD). |
| Medium： | ATCC® Medium 2107: Modified Reinforced Clostridial |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Anaerobic |
| Name of Depositor： | FR Fekety |
| Special Collection： | NCRR Contract |
| Year of Origin： | 1977 |
| References： | Fekety R, et al. The hunt for Clostridium difficile: 21-year follow-up of a stool specimen sent for culture. Lancet 354: 1115-1116, 1999. PubMed: 10509517 F. R Fekety, personal communication Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986 |