| 产品名称: | pDP97 |
|---|---|
| 商品货号: | TS162258 |
| Designations: | pDP97 |
| Species: | Homo sapiens, human |
| Vector: | Construct size (kb): 8.0 |
| Insert: | DNA: genomic Insert lengths(kb): 5.300000190734863 Gene product: DNA Segment, repetitive DYZ3 |
| Insert Size (kb): | 5.300 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 2.7; HindIII--8.3; BamHI--8.3; PstI--4.5, 2.8, 0.57; AccI--5.0, 2.96. Cross-hybridizes weakly to alphoid repeats on chromosomes 13, 14, and 15. Hybridize at high stringency (50 C, 50% formamide) to avoid cross-hybridization. Also a marker for deletion interval 4B. Shows Y specificity, but at high stringency it detects a repeated Y specific EcoRI fragment of 5.5 kb (DXZ3). |
| References: | Disteche CM, et al. Molecular detection of a translocation (Y;15) in a 45,X male. Hum. Genet. 74: 372-377, 1986. PubMed: 3793100 Page DC. Sex reversal: deletion mapping the male-determining function of the human Y chromosome. Cold Spring Harbor Symp. Quant. Biol. 51: 229-235, 1986. PubMed: 3472719 Wolfe J, et al. Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome. J. Mol. Biol. 182: 477-485, 1985. PubMed: 4040175 David C Page, personal communication |