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| 产品名称： | pBSV2 |
|---|---|
| 商品货号： | TS162381 |
| Designations： | pBSV2 |
| Depositors： | PE Stewart |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 6.4000000953674320 DESCRIPTION OF VECTOR: Intact vector size: 6.400 Type of vector: plasmid Cloning sites: NotI SacI EcoRI KpnI BamHI XbaI SalI PstI SphI HindIII NotI Polylinker sites: NotI SacI EcoRI KpnI BamHI XbaI SalI PstI SphI HindIII NotI Other unique sites: NcoI SfiI SpeI Construction: pCR-XL-TOPO Features (with orientation and position when available): primer site: M13 (forward, reverse) promoter: lacZalpha replicon: ColE1, ORFs marker(s): kanamycin, zeocin Vector: pBSV2 (plasmid) Promoters: Promoter lacZalpha Construction: pCR-XL-TOPO Marker(s):kanR Construct size (kb): 6.400000095367432 Features: marker(s): kanamycin, zeocin primer site: M13 (forward, reverse) promoter: lacZalpha replicon: ColE1, ORFs |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.4; EcoRI--4.2, 2.2; HindIII--6.4. The plasmid pBSV2 is a shuttle vector capable of moving between E. coli and the Lyme disease spirochete, Borrelia burgdorferi. |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stewart P, et al. Isolation of a circular plasmid region sufficient for autonomous replication and transformation of infectious Borrelia burgdorferi. Mol. Microbiol. 39: 714-721, 2001. PubMed: 11169111 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |