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| 产品名称： | pKSM714 |
|---|---|
| 商品货号： | TS162438 |
| Designations： | pKSM714 |
| Depositors： | K Ippen-Ihler, S Maneewannakul |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 5.0409998893737790 DESCRIPTION OF VECTOR: Intact vector size: 5.041 Type of vector: phagemid Cloning sites: BamHI HindIII XbaI AvaI SmaI EcoRI Polylinker sites: BamHI HindIII XbaI AvaI SmaI EcoRI Construction: pKSM710, pSM102 Host range: Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: T7lac, ->, 20-36 promoter for expression: lacUV5, ->, 197-226 MCS: BamHI...EcoRI, ->, 340-391 insert detection: lacZalpha, ->, 335-607 transcription terminator: T7, ->, 609-650 transcription terminator: rrnB, ->, 707-750 transcription terminator: rrnB, ->, 881-910 transcription terminator: fd, ->, 1006-1052 transcription terminator: fd, ->, 1116-1162 replicon: fd, ->, 1295-1750 replicon: pMB1, repressor gene: lacIq, ->, 2666-3748 marker(s): kanR, ->, 4174-4968 terminator: trpA, ->, 4989-5016 Vector: pKSM714 (phagemid) Promoters: Promoter for in vitro transcription T7lac Construction: pKSM710, pSM102 Marker(s):kanR Construct size (kb): 5.040999889373779 Features: insert detection: lacZalpha marker(s): kanR promoter for expression: lacUV5 promoter for in vitro transcription: T7lac replicon: fd replicon: pMB1 repressor gene: lacIq MCS: BamHI...EcoRI terminator: trpA transcription terminator: T7 transcription terminator: fd transcription terminator: rrnB |
| Applications： | expression vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.2, 0.65. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--4.14; EcoRI--0.39; KpnI--0.36, 2.59; NcoI--0.33, 4.74; PstI--4.35, 5.03; PvuI--0.51; PvuII--0.48, 3.62, 3.71, 4.05, 4.41; SacI--0.38, 2.58; SalI--5.04. Differs from pKSM713 (ATCC 87159) only in the orientation of the pSM102 fragment containing lacIq, kanR and the trpA terminator. Expression vector. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |