| 产品名称: | pSH2-gamma3 |
|---|---|
| 商品货号: | TS162470 |
| Designations: | pSH2-gamma3 |
| Species: | Homo sapiens, human |
| Depositors: | M Lefranc |
| Vector: | Construct size (kb): 13.0 |
| Insert: | DNA: genomic Insert lengths(kb): 7.300000190734863 Gene product: immunoglobulin gamma 3 (Gm marker) IGHG3 Alleles: A1, A2, A3, A4, A5, A6 |
| Insert Size (kb): | 7.300 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Insert contains internal SphI (2), KpnI (1) and SstI (1) sites. Restriction digests of the clone give the following sizes (kb): HindIII--7.8, 4.85; PstI--4.2, 2.45, 1.8, 1.65, 0.69; EcoRI--13.0; BamHI--13.0, 0.5; SalI--13.0. This plasmid shows some instability. We recommend growing on LB agar with ampicillin, and picking colonies to select the correct clone. Contains the complete gene. |
| References: | Huck S, et al. Sequence of a human immunoglobulin gamma 3 heavy chain constant region gene: comparison with the other human C gamma genes. Nucleic Acids Res. 14: 1779-1789, 1986. PubMed: 3081877 Huck S, et al. A gamma 3 hinge region probe: first specific human immunoglobulin subclass probe. FEBS Lett. 208: 221-230, 1986. PubMed: 2877902 Marie-Paule Lefranc, personal communication |