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| 产品名称： | cHuVim2 cHuVIM2 |
|---|---|
| 商品货号： | TS162526 |
| Designations： | cHuVim2 cHuVIM2 |
| Species： | Homo sapiens, human |
| Applications： | Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences. |
| Vector： | Construct size (kb): 3.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.100000023841858 Tissue: fibroblast Gene product: vimentin VIM Alleles: A1, A2 |
| Insert Size (kb)： | 1.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Depositor indicates digests give (kb): EcoRI--2.7, 0.9; HincII--2.7, 0.9; PstI--3, 0.4, 0.2; BamHI--3.6; EcoRI+HincII--2.7, 0.9. Insert contains the following sites separated by (bp) (approx): EcoRI- 35- MspI- 35- HincII- 95- PstI- 115- PstI- 100- MspI- 140- PstI- 140- MspI- 335- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--2.85, 0.95; HincII--3.05, 0.92; EcoRI/HincII--2.95, 0.92; PstI--3.2, 0.44, 0.32, 0.21; BamHI--3.9. Insert begins at nt 561 (corresponding to amino acid 187) after the ATG start and extends 157 nt after the stop codon. This probe contains exon 4. Shows 90% homology with coding region of hamster vimentin. Positions of non-coding regions conserved, but less than 45% homology between the human and hamster intron sequences. |
| References： | Perreau J, et al. Nucleotide sequence of the human vimentin gene and regulation of its transcription in tissues and cultured cells. Gene 62: 7-16, 1988. PubMed: 3371665 Denise Paulin, personal communication |