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微信小章| 产品名称： | pCMM66 |
|---|---|
| 商品货号： | TS162554 |
| Designations： | pCMM66 |
| Species： | Homo sapiens, human |
| Applications： | A 1.8 kb EcoRI/HindIII fragment has been used to detect the PstI polymorphism. Otherwise, RFLPs were observed under normal hybridization and wash stringencies. |
| Vector： | Construct size (kb): 7.099999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 4.800000190734863 Gene product: DNA Segment, single copy D14S22 |
| Insert Size (kb)： | 4.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.55, 1.75, 1.55, 1.2; EcoRI--5.2, 1.65; AccI--4.1, 2.7, consistent with deposited DNA. Codominant segregation demonstrated in 34 3-generation families for the PstI RFLP. A 1.8 kb EcoRI/HindIII fragment has been used to detect the PstI polymorphism. The probe was pre-associated with excess human DNA before hybridization. Otherwise, RFLPs were observed under normal hybridization and wash stringencies. Enzyme(s) not detecting polymorphism: MspI, TaqI. |
| References： | Wang JC, et al. Uniparental heterodisomy for chromosome 14 in a phenotypically abnormal familial balanced 13/14 Robertsonian translocation carrier. Am. J. Hum. Genet. 48: 1069-1074, 1991. PubMed: 2035528 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pCMM66) on chromosome 14 D14S22. Nucleic Acids Res. 16: 6255, 1988. PubMed: 2899877 |