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微信小章| 产品名称： | PEAKrapid |
|---|---|
| 商品货号： | TS162579 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Cell Type： | transformed with adenovirus 5 DNA |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | loosely adherent |
| Biosafety Level： | 2 cells containing Adenovirus type 5 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus |
| Applications： | The cells are useful for protein expression. They support the replication of recombinant plasmids with the Epstein-Barr virus (EBV) oriP or SV40 origin of replication. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | This line was derived from the human embryonic kidney line, HEK 293, ATCC CRL-1573.xa0 |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.05 mg/ml Gentamicin sulfate and 10% fetal bovine serum.
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| Subculturing： | Volumes used in this protocol are for 75 cm2xa0flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Interval: Maintain cultures at a cell concentration between 8 x 103xa0and 1 x 105xa0cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every two to three days
Note:For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature:xa037°C |
| Population Doubling Time： | 26 hours |
| Name of Depositor： | J Seed |
| References： | Nelson G, et al. An amino-acid taste receptor. Nature 416: 199-202, 2002. PubMed: 11894099 Magistrelli G, et al. Rapid, simple and high yield production of recombinant proteins in mammalian cells using a versatile episomal system. Protein Expression and Purification: 72(2): 209-216, 2010. PubMed: 20399863 Dobi A, et al. Mammalian expression cloning of nucleic acid binding proteins by agarose thin-layer gelshift clone selection. Biotechniques: 33(4): 868-872, 2002. PubMed: 12398195 |