| 产品名称: | pCW7 plasmid in E. coli |
|---|---|
| 商品货号: | TS162617 |
| Designations: | pCW7 plasmid in E. coli |
| Species: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors: | R. Kopito |
| Vector: | Construct size (kb): 4.136 Vector: pRBG4 Vector size (Kb): 3.806 |
| Insert: | Ubiquitin, wild type Insert size: 0.330 (ubiquitin, 6 HIS and c-myc tag) Insert site: ClaI/EcoRI Excise insert: EcoRI |
| Putative ID: | ubiquitin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | This is s cDNA clone of wild type ubiquitin from Saccharomyces cerevisiae with a 6 HIS, c-myc N-terminal tag cloned into the ClaI/EcoRI sites of the pRBG4 vector. The plasmid was transformed into E. coli DH10B. |
| Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References: | Ward CL, et al. Degradation of CFTR by the ubiquitin-proteasome pathway. Cell 83: 121-127, 1995. |