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| 产品名称： | pMMB190 |
|---|---|
| 商品货号： | TS162721 |
| Designations： | pMMB190 |
| Depositors： | VM Morales |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.0640001296997070 Vector: pMMB190 (plasmid) Promoters: Promoter tac Construction: pMMB66EH, pUC8 Marker(s):ampR Construct size (kb): 9.064000129699707 Features: insert detection: lacZ marker(s): ampR promoter: tac, lacUV5 replicon: oriV, oriT (IncQ) repressor gene: lacIq transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.1; EcoRV--5.3, 3.8; SspI--4.9, 1.8, 1.6, 1.0; EcoRI/MluI--8.2, 0.9. Contains sequences encoding lacIq, a tandem tac lacUV5 promoter region, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Shows a low level of expression without induction. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli xa0containing the plasmid. |