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微信小章| 产品名称： | Euplotes gracilis |
|---|---|
| 商品货号： | TS162893 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | test tube |
| Type Strain： | no |
| Cryopreservation： | Cryoprotective Solution 10% (v/v) sterile DMSO Fresh growth medium w/o bacteria 1.xa0xa0xa0xa0 Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat. Allow to cool before use. 2.xa0xa0xa0xa0 Harvest cells from a culture that is at or near peak density by filtration and/or centrifugation at the lowest level and shortest time required to loosely pellet cells (for this strain, typically 300-500 x g for 3-5 min). 3.xa0xa0xa0xa0 Adjust the concentration of cells at least 2 x 105/ml in fresh medium. 4.xa0xa0xa0xa0 Mix the cell preparation and the cryoprotective solution in equal portions. 5.xa0xa0xa0xa0 Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6.xa0xa0xa0xa0 Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. 7.xa0xa0xa0xa0 Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator. 8.xa0xa0xa0xa0 To establish a culture from the frozen state place a vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial. 9.xa0xa0xa0xa0 Immediately after thawing, do not leave in water bath, aseptically transfer the contents of the ampule onto the surface of a 20 x 100 mm agar plate of ATCC medium 919 (non-nutrient agar) containing an overlay of 15.0 ml of ATCC medium 1667 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC® 700831) or Enterobacter aerogenes (ATCC® 13048). 10.xa0 Incubate the plate at 20-25°C. 11.xa0 Once the culture is established, transfer 0.5 - 1.0 ml to a T-25 tissue culture flask or 16 x 125 mm test tube containing 5 ml bacterized ATCC medium 1667. 12.xa0 Incubate at 20-25°C (incubate a test tube upright with the cap on loosely).xa0 Follow the protocol for maintenance of culture. |
| Name of Depositor： | BA Hill |