微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pCM190 |
|---|---|
| 商品货号： | TS162895 |
| Designations： | pCM190 |
| Depositors： | E Herrero |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.0120000839233400 Vector: pCM190 (plasmid) Promoters: Promoter CMV Marker(s):URA3,ampR Construct size (kb): 8.01200008392334 Features: marker(s): URA3 marker(s): ampR operator: tetO - seven repeats other: tTA transcriptional activator promoter: CMV promoter: CYC1 replicon: 2 micron replicon: pMB1 MCS: BamHI...PstI terminator: ADH1 terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.0; PstI--8.0; BamHI/XhoI--7.2, 0.7. Yeast episomal expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672 |
| Related Products： | component of:ATCC 87663 |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of Escherichia coli DH5alpha containing the plasmid. |