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| 产品名称： | pVY3 |
|---|---|
| 商品货号： | TS162924 |
| Designations： | pVY3 |
| GenBank Number： | U08629 |
| Species： | Yamadazyma stipitis (Pignal) Billon-Grand, teleomorph |
| Depositors： | TW Jeffries |
| Vector： | Construct size (kb): 4.407999992370606 |
| Insert： | DNA: genomic Insert lengths(kb): 1.445999979972839 Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5-phosphate decarboxylase, orotate phosphoribosyltransferase 1) URA3 |
| Insert Size (kb)： | 1.446 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--4.8; SalI/XbaI--3.2, 1.6; XbaI--4.8. The insert contains the following restriction sites (approximate kb from the 5 end): AccI--0.00, 0.62, 0.78; HindIII--0.16; KpnI--0.51; EcoRI--1.20. |
| References： | Yang VW, et al. High-efficiency transformation of Pichia stipitis based on its URA3 gene and a homologous autonomous replication sequence, ARS2. Appl. Environ. Microbiol. 60: 4245-4254, 1994. PubMed: 7811063 |