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| 产品名称： | pJJ281 pJJ248 |
|---|---|
| 商品货号： | TS162936 |
| Designations： | pJJ281 pJJ248 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Prakash |
| Vector： | Construct size (kb): 3.700000047683716 |
| Insert： | DNA: genomic Insert lengths(kb): 0.9359999895095825 Gene product: phosphoribosylanthranilate isomerase TRP1 |
| Insert Size (kb)： | 0.936 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--2.7, 0.7, 0.4; PvuII--2.4, 1.3; EcoRI--3.7. A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions. Constructed by filling in the ends of both the vector and the insert fragment. TRP1 is in the same orientation as lacZ. The insert was subcloned from YRp17. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072 Louise Prakash, personal communication |