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| 产品名称： | pKi-67/1 |
|---|---|
| 商品货号： | TS163006 |
| Designations： | pKi-67/1 |
| GenBank Number： | X65550 |
| Species： | Homo sapiens, human |
| Depositors： | J Gerdes |
| Vector： | Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR325 Intact vector size: 5.996 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI EcoRV (MstI-Cm?) NcoI PstI PvuI SalI SphI XmaIII XmnI Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, cmLR, tetR replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MKI67 Gene name: antigen identified by monoclonal antibody Ki-67 Contains complete coding sequence?: N Chromosome: 10; Localization: 10 q25-qter Tissue: IM-9 cell line Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 1.092 Cross references: DNA Seq. Acc.: X65550 Insert lengths(kb): 1.092000007629395 Tissue: IM-9 cell line Gene product: antigen identified by monoclonal antibody Ki-67( Proliferation-related Ki-67 antigen, Ki-67 antigen) MKI67 |
| Insert Size (kb)： | 1.092 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 1.1; HindIII--7.0; PstI--5.0, 2.1; SacI--7.1; XbaI--7.1. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--1.00; SacI--0.21, 0.57, 0.73, 0.94; XbaI--0.18. It includes three of sixteen Ki-67 repeats. Detects splicing isoforms of 8.7 and 9.8 kb. |
| References： | Fonatsch C, et al. Assignment of the human Ki-67 gene (MKI67) to 10q25-qter. Genomics 11: 476-477, 1991. PubMed: 1769665 |