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| 产品名称： | pHPT6 |
|---|---|
| 商品货号： | TS163028 |
| Designations： | pHPT6 |
| Species： | Mus musculus, mouse |
| Depositors： | C Caskey |
| Vector： | Construct size (kb): 5.75 |
| Insert： | DNA: genomic Insert lengths(kb): 1.389999985694885 Tissue: myeloma Gene product: hypoxanthine phosphoribosyltransferase Hprt |
| Insert Size (kb)： | 1.390 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Depositor indicates the following restriction digests (kb): EcoRI--4.3, 1.39; EcoRI+BglII--4.3, 1.18, 0.207; AvaI--2.5, 3.25; AccI--3.12, 1.6, 1.07; AvaII--1.75 (doublet), 1.07, several<0.3; EcoRI+AvaII--1.75, 0.96, 0.8, 0.63, 0.4, several smaller. Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 1.39; HindIII--6.2. The insert is a subclone of lambdaHPT23 (ATCC 37598) and contains the promoter and exon 1 of the gene (105 bp of 5 untranslated sequence plus sequence coding for the first 9 amino acids). |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Melton DW, et al. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc. Natl. Acad. Sci. USA 81: 2147-2151, 1984. PubMed: 6326107 C. Thomas Caskey, personal communication |