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| 产品名称： | pSCH2011 |
|---|---|
| 商品货号： | TS163062 |
| Designations： | pSCH2011 |
| GenBank Number： | V01547 |
| Species： | Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz |
| Depositors： | KJ Shaw |
| Vector： | Construct size (kb): 3.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.5630000233650208 Gene product: 35"-aminoglycoside phosphotransferase (type III) aph(3)-IIIa |
| Insert Size (kb)： | 0.563 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 0.5; EcoRI--3.5; HindIII--3.5. A single gel purification of the PCR generated probe is necessary since flanking regions will co-amplify with the gene specific sequence. Failure to do so often results in high backgrounds and false positives with clinical E. coli strains. A hybridization probe may be generated using the following vector specific PCR primers: modified T3 = 5-CCCCTCACTAAAGGGAACAAAAGCTG-3 and modified T7 = 5-CGCGTAATACGACTCACTATAGGGCGAA-3. |
| References： | Shaw KJ, et alThe application of molecular techniques for the study of aminoglycoside resistanceIn: Shaw KJ, et alMethods in molecular medicine: molecular approaches for the diagnosis and investigation of bacterial diseasesTotowa, NJHumana Presssubmitted, 1996 |